Simian-human HAV having a chimeric 2C protein
AssigneeThe United States of America as represented by the Department of Health and Human Services
InventorsGopa Raychaudhuri, Suzanne U. Emerson, Robert H. Purcell
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Abstract
The present invention discloses simian-human hepatitis A virus chimeric genomes which encode a hepatitis A virus having a chimeric 2C protein. The invention further discloses the use of these viruses or the nucleic acid sequence encoding them as vaccines.
Claims33
A DNA construct comprising a whole human attenuated hepatitis A virus (HAV) genome sequence wherein at least one fragment of the 2C gene of the human attenuated genome sequence is replaced by the corresponding fragment(s) of the 2C gene of a simian hepatitis A virus AGM-27 genome sequence, wherein the AGM-27 2C gene fragment does not comprise the entire 2C gene and encodes an amino acid sequence which differs in at least one amino acid residue from the amino acid sequence encoded by the corresponding fragment of the 2C gene of the human attenuated hepatitis A virus.
A hepatitis A virus having a genome comprising a human attenuated hepatitis A virus genome in which at least one fragment of the 2C gene of the attenuated hepatitis A virus genome has been replaced by a corresponding fragment of a 2C gene from a simian AGM-27 hepatitis A virus genome sequence, wherein the AGM-27 2C gene fragment does not comprise the entire 2C gene and encodes an amino acid sequence which differs in at least one amino acid residue from the amino acid sequence encoded by the corresponding fragment of the 2C gene of the human attenuated HAV.
Dependencies are inferred from the claim text.