Compositions containing thrombin and microfibrillar collagen and methods for preparation and use thereof
AssigneeCohesion Technologies, Inc.
InventorsJeffrey J. Prior, Donald G. Wallace, David H. Sierra, Frank A. DeLustro
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Abstract
The present invention relates to thrombin-containing hemostatic compositions, their preparation and use. In particular, it relates to hemostatic compositions comprising stabilized thrombin and microfibrillar collagen in an aqueous medium. In a preferred embodiment of the present invention, the compositions are used in a kit comprising two different components, one of which is autologous patient's plasma as the source of fibrinogen, and the other of which is the thrombin-containing composition which also contains microfibrillar collagen.
Claims42
A hemostatic composition comprising thrombin and microfibrillar collagen in an aqueous medium, wherein the microfibrillar collagen has an average diameter of about 3-30 nm.
A method for promoting hemostasis of a tissue site on or in a body of a mammalian subject comprising the steps of:
- (a) providing a hemostatic composition comprising thrombin and microfibrillar collagen in an aqueous medium, wherein the microfibrillar collagen has an average diameter of about 3-30 mn;
- (b) providing an aqueous medium comprising fibrinogen;
- (c) mixing the hemostatic composition and the aqueous medium comprising fibrinogen immediately prior to contact with the tissue site;
- and
- (d) contacting the mixture with the tissue site.
A method for promoting hemostasis of a tissue site on or in a body of a mammalian subject comprising the steps of:
- (a) providing a hemostatic composition comprising thrombin and microfibrillar collagen in an aqueous medium, wherein the microfibrillar collagen has an average diameter of about 3-30 nm;
- (b) providing an aqueous medium comprising platelets;
- (c) mixing the hemostatic composition and the aqueous medium comprising platelets immediately prior to contact with the tissue site;
- and
- (d) contacting the mixture with the tissue site.
A method of making a composition for use as a hemostatic agent comprising the steps of:
- (a) mixing thrombin, fibrillar collagen and a fiber disassembly agent together in an aqueous medium, wherein the fiber disassembly agent is present at a concentration which is effective at disassembling the fibrillar collagen into microfibrillar collagen;
- and
- (b) incubating the mixture formed in step (a) for a sufficient length of time and at a sufficient temperature to form microfibrillar collagen having a stable fiber size wherein the stable fiber size is about 3-30 nm in average diameter.
Dependencies are inferred from the claim text.