Production of Bacillus thuringiensis integrants
AssigneeValent BioSciences Corporation
InventorsLee Fremont Adams, Michael David Thomas, Alan P. Sloma, William R. Widner, Steen Troels Jørgensen, Per Linå Jørgensen, Børge Krag Diderichsen
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Abstract
The invention relates to a method for producing an integrant(s) of Bacillus thuringiensis. The invention further relates to such integrants, compositions comprising such integrants, as well as methods for controlling a pest(s) using these compositions.
Claims10
An integrant of Bacillus thuringiensis or spore thereof which produces at least one heterologous crystal delta-endotoxin, wherein said heterologous crystal endotoxin is produced from a homologously recombined gene in said integrant's chromosome, and wherein said integrant has greater pesticidal activity than a corresponding parental strain by producing a larger quantity of a crystal delta-endotoxin as compared to said corresponding parental strain.
A DNA construct comprising (a) an origin of replication but lacking a functional gene or portion thereof encoding a factor required for plasmid replication from the origin of replication;
- (b) a DNA sequence encoding a Bacillus thuringiensis delta-endotoxin;
- (c) a DNA sequence that is homologous with a region of the genome of a Bacillus thuringiensis;
- and (d) a selectable marker.
Dependencies are inferred from the claim text.