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AssigneesValant BioSciences, Inc., Libertyville, Inc.
InventorsLee Fremont Adams, Michael David Thomas, Alan P. Sloma, William R. Widner
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The invention relates to a method for producing an integrant(s) of Bacillus thuringiensis which produces a larger quantity of a crystal delta-endotoxin greater pesticidal activity as compared to the crystal delta-endotoxin produced by the corresponding parental strain. The crystal delta-endotoxin produced by the integrant Bacillus thuringiensis will have an activity directed towards the same pest(s) as its parent Bacillus thuringiensis crystal delta-endotoxin. The invention further relates to such integrants, compositions comprising such integrants, as well as methods for controlling a pest(s) using these compositions.
An integrant of Bacillus thuringiensis or spore thereof which produces at least one homologous or heterologous crystal delta-endotoxin, wherein said homologous or heterologous crystal delta-endotoxin is produced form a homologously recombined gene in said integrant's chromosome, and wherein said integrant has greater pesticidal activity than a corresponding parental strain by producing a larger quantity of a crystal delta-endotoxin as compared to said corresponding parental strain.
A DNA construct lacking a Bacillus thuringiensis origin of replication comprising (i) a DNA sequence encoding a delta-endotoxin wherein said DNA sequence is obtained from a Bacillus thuringiensis strain producing larger amounts of said delta-endotoxin than a parental strain producing said delta-endotoxin;
Dependencies are inferred from the claim text.