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US 6,277,608 B1Granted
Recombinational cloning using nucleic acids having recombination sites
AssigneeInvitrogen Corporation
InventorsJames L. Hartley, Michael A. Brasch, Gary F. Temple, Donna K. Fox
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Abstract
Recombinational cloning is provided by the use of nucleic acids, vectors and methods, in vitro and in vivo, for moving or exchanging segments of DNA molecules using engineered recombination sites and recombination proteins to provide chimeric DNA molecules that have the desired characteristic(s) and/or DNA segment(s).
Claims23
A method for cloning or subcloning desired nucleic acid molecules comprising
- a) combining in vitro or in vivo
- i) one or more Insert Donor molecules comprising one or more nucleic acid segments flanked by two or more recombination sites, wherein said recombination sites do not substantially recombine with each other;
- ii) two or more different Vector Donor molecules, each comprising two or more recombination sites, wherein said recombination sites do not substantially recombine with each other;
- and
- iii) one or more site specific recombination proteins;
- and
- b) incubating the combination under conditions sufficient to transfer one or more of said nucleic acid segments into said different Vector Donor molecules, thereby producing two or more different Product molecules.
4 more elements
Dependencies are inferred from the claim text.