Method and system for performing infrared study on a biological sample
AssigneesVysis, Inc., The Procter & Gamble Company
InventorsRina K. Dukor, Curtis A. Marcott
Life of this patent
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Abstract
A method and system performs Fourier transform infrared (FT-IR) imaging microspectroscopy on a biological sample fixed on a substrate with a supporting surface that generally reflects infrared light while generally transmitting visible light. Infrared light impinging on the biological sample is reflected by the supporting surface of the substrate. Infrared light from the sample is focused onto a focal-plane array detector with multiple pixels for detecting infrared images of the sample. The detected infrared images are processed to generate spectral images of the sample. The same biological sample is suitable for conventional pathological studies.
Claims29
A method of examining biological samples comprising the steps of:
- positioning a biological sample supported on a supporting surface at an imaging position, the supporting surface generally reflecting infrared light while generally transmitting visible light;
- directing an infrared beam in the mid-infrared range to the biological sample;
- collecting infrared images of the biological sample in a reflection-absorption mode by detecting infrared light reflected by the supporting surface and through the biological sample with a detector array having multiple detection pixels;
- and
- deriving a Fourier transform infrared (FT-IR) spectrum from the collected infrared images.
A method of identifying cellular types in biological samples comprising the step of:
- positioning a biological sample supported on a supporting surface at an imaging position, the supporting surface being generally reflective of infrared light while generally transmitting visible light;
- inspecting the biological sample using visible light to identify a site of interest in the sample;
- positioning the site of interest for infrared imaging with a Fourier transform infrared (FT-IR) imaging device, the FT-IR imaging device including an array detector having multiple detection pixels;
- collecting infrared images of the site of interest in a reflection-absorption mode by directing an infrared beam in the mid-infrared range to the site of interest and detecting infrared light reflected by the supporting surface and through the biological sample from the site of interest;
- and
- deriving an infrared spectrum from the collected infrared images for identifying cellular types at the site of interest.
A system for studying biological samples comprising:
- a biological sample supported on a supporting surface generally reflecting infrared light and generally transmitting visible light;
- a Fourier transform infrared (FT-IR) spectrometer for generating an input infrared beam in the mid-infrared range for illuminating the biological sample;
- a focal-plane array detector having multiple detection pixels for detecting infrared images of the biological sample for derivation of spectral images of the sample;
- and
- infrared optical elements for imaging the sample in a reflection-absorption mode wherein the infrared optical elements focuses infrared light reflected by the supporting surface and through the biological sample to the focal-plane array detector.
A method of examining biological samples comprising the steps of:
- positioning a biological sample supported on a supporting surface at an imaging position, the supporting surface generally reflecting infrared light;
- directing an infrared beam in the mid-infrared range to the biological sample;
- collecting infrared images of the biological sample in a reflection-absorption mode by detecting infrared light reflected by the supporting surface and through the biological sample with a detector array having multiple detection pixels;
- and
- deriving a Fourier transform infrared (FT-IR) spectrum from the collected infrared images.
Dependencies are inferred from the claim text.