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US 6,274,791 B1Granted
Methods for strawberry transformation using Agrobacterium tumefaciens
Assignee(VPP Corporation) DNA Plant Technology Corporation
InventorsSeema Dhir, Maud A. W. Hinchee, Jeanne G Layton, Janette V. Oakes
Life of this patent
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Abstract
Methods for Agrobacterium-mediated transformation and regeneration of strawberry plants are disclosed. Substitution of glucose or fructose for sucrose in various media preparations results in improved transformation efficiencies.
Claims20
A method of preparing transgenic strawberry shoots, the method comprising:
- (a) contacting strawberry explants with Agrobacterium tumefaciens in a co-cultivation medium prepared using glucose or fructose, thereby producing transformed strawberry explants;
- and
- (b) culturing the transformed strawberry explants in a selection medium prepared using glucose or fructose, thereby producing transformed strawberry shoots.
Dependencies are inferred from the claim text.