Nucleic acids encoding a plant enzyme involved in very long chain fatty acid synthesis
AssigneeThe University of British Columbia
InventorsLjerka Kunst, Anthony A. Millar
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Abstract
Nucleic acid molecules encoding an enzyme involved in very long chain fatty acid (VLCFA) elongation in plants are disclosed. The invention includes a cDNA, genomic clone and encoded protein, as well as plants having modified VLCFA composition, such as modified epicuticular waxes, and methods of making such plants.
Claims12
A recombinant nucleic acid molecule comprising a promoter sequence operably linked to a nucleic acid sequence, wherein the promoter sequence comprises a transcriptional regulatory region capable of mediating gene expression in epidermal cells of Arabidopsis wherein the transcriptional regulatory region hybridezes under stringent conditions to:
- Seq. I.D. No. 12 or the complement of Seq. I.D. No. 12.
A method of isolating a nucleic acid molecule having promoter activity, comprising hybridizing under stringent conditions a nucleic acid preparation with a probe comprising Seq. I.D. No. 12 or the complement of Seq. I.D. No. 12.
A recombinant nucleic acid molecule comprising a promoter sequence operably linked to a nucleic acid sequence, wherein the promoter sequence comprises a transcriptional regulatory region capable of mediating gene expression in epidermal cells of Arabidopsis wherein the transcriptional regulatory region is obtainable from a plant VLCFA condensing enzyme gene comprising an open reading frame that hybridizes under stringent conditions to Seq. I.D. No. 3 or to the complement of Seq. I.D. No. 3.
Dependencies are inferred from the claim text.