Gcp
AssigneeSmithKline Beecham Corporation
InventorsSanjoy Biswas, Alison Frances Chalker, David Holmes, Karen A Ingraham, Leslie Marie Palmer, Jennifer E Ray, Richard Lloyd Warren, Magdalena Zalacain
Life of this patent
Estimating the term…
Abstract
The invention provides gcp polypeptides and polynucleotides encoding gcp polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing gcp polypeptides to screen for antibacterial compounds.
Claims21
An isolated polynucleotide segment comprising a first polynucleotide sequence or the full complement of the entire length of the first polynucleotide sequence, wherein the first polynucleotide sequence is identical to SEQ ID NO:
- 1, except that, over the entire length corresponding to SEQ ID NO:1, nn nucleotides are substituted, inserted or deleted, wherein nn satisfies the following expression
- nn≦xn−(xn·y)
- wherein xn is the total number of nucleotides in SEQ ID NO:1, y is at least 0.95, and wherein any non-integer product of xn and y is rounded down to the nearest integer before subtracting the product from xn;
- and wherein the first polynucleotide sequence detects Streptococcus pneumoniae.
An isolated polynucleotide segment, comprising a first polynucleotide sequence or the full complement of the entire length of the first polynucleotide sequence, wherein the first polynucleotide sequence comprises SEQ ID NO:
- 1.
An isolated polynucleotide segment, comprising a first polynucleotide sequence or the full complement of the entire length of the first polynucleotide sequence, wherein the first polynucleotide sequence hybridizes to the full complement of SEQ ID NO:1, wherein the hybridization conditions include incubation at 42° C. in a solution comprising:
- 50% formamide, 5×SSC (150 mM NaCl, 15 mM trisodium citrate), 50 mM sodium phosphate (pH7.6), 5×Denhardt's solution, 10% dextran sulfate, and 20 micrograms/ml denatured, sheared salmon sperm DNA, followed by washing in 0.1×SSC at 65° C.;
- and, wherein the first polynucleotide sequence is identical to SEQ ID NO:1, except that, over the entire length corresponding to SEQ ID NO:1, nn nucleotides are substituted, inserted or deleted, wherein nn satisfies the following expression
- nn≦xn−(xn·y)
- wherein xn is the total number of nucleotides in SEQ ID NO:1, y is at least 0.95, and wherein any non-integer product of xn and y is rounded down to the nearest integer before subtracting the product from xn;
- and wherein the first polynucleotide sequence detects Streptococcus pneumoniae.
An isolated polynucleotide segment comprising a first polynucleotide sequence or the full complement of the entire length of the first polynucleotide sequence, wherein the first polynucleotide sequence encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:2.
An isolated polynucleotide segment comprising a first polynucleotide sequence or the full complement of the entire length of the first polynucleotide sequence, wherein the first polynucleotide sequence encodes a polypeptide consisting of the amino acid sequence set forth in SEQ ID NO:2.
Dependencies are inferred from the claim text.