Interferon-gamma (IFN-γ) inducing factor (IGIF, IL-18) and peptide fragment thereof
AssigneeKabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
InventorsHaruki Okamura, Tadao Tanimoto, Kakuji Torigoe, Toshio Kunikata, Mutsuko Taniguchi, Keizo Kohno, Masashi Kurimoto
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Abstract
A protein which induces the IFN-γ production by immunocompetent cells and has a molecular weight of 19,000±5,000 daltons on SDS-PAGE or gel filtration method and a pI of 4.8±1.0 on chromatofocusing. The protein is isolated from mouse liver and can be purified by a monoclonal antibody specific to it. The monoclonal antibody can be also used for assaying the protein.
Claims9
An isolated peptide fragment obtainable from a polypeptide of SEQ ID NO:2, where Xaa at residue 70 is methionine or threonine, and capable of being used as an antigen to which a monoclonal antibody is prepared, wherein said polypeptide of SEQ ID NO:2 is capable of inducing IFN-γ production by immunocompetent cells and has a molecular weight of about 19,000±5,000 daltons on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and an isoelectric point of about 4.8±1.0 on chromatofocusing.
An isolated collection of peptide fragments obtainable from a polypeptide of SEQ ID NO:2, where Xaa at residue 70 is methionine or threonine, and capable of being used as antigens to which monoclonal antibodies are prepared, wherein said polypeptide of SEQ ID NO:2 is capable of inducing IFN-γ production by immunocompetent cells and having a molecular weight of about 19,000±5,000 daltons on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and an isoelectric point of about 4.8±1.0 on chromatofocusing.
A purified protein comprising an amino acid sequence selected from the group consisting of SEQ ID NO:2, where Xaa at residue 70 of SEQ ID NO:2 is methionine or threonine, and a variant of SEQ ID NO:2 in which one or more amino acid residues are deleted from the N-terminus or one amino acid residue is replaced with a different amino acid residue, but which retains the capability of the protein comprising SEQ ID NO:2 to induce IFN-γ production by immunocompetent cells.
Dependencies are inferred from the claim text.