Method of inhibiting the proliferation and causing the differentiation of cells with IGF-1 receptor antisense oligonucleotides
AssigneeThomas Jefferson University
InventorsRenato Baserga, Christian Sell, Raphael Rubin
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Abstract
A method of inhibiting the proliferation and causing the differentiation of undifferentiated cells comprising contacting the undifferentiated cells with an effective amount of an antisense oligonucleotide having a sequence which is complementary to a region of the IGF-1 receptor RNA. The sequence of the antisense oligonucleotide is selected from an oligodeoxynucleotide sequence complementary to codons −29 to −24 of the signal sequence of the IGF-1 receptor and an oligoribodeoxynucleotide sequence complementary to codons 1 to 309 of the sequence of the IGF-1 receptor. The oligoribodeoxynucleotide sequence may be provided by an expression vector.
Claims14
A method of inhibiting proliferation of undifferentiated cells in a mammal in vivo comprising:
- contacting an undifferentiated cell ex vivo with an effective amount of an oligonucleotide substantially complementary to a region of human IGF-1 receptor RNA, wherein said IGF-1 receptor RNA expression is inhibited;
- and
- administering said undifferentiated cell treated ex vivo to said mammal.
A method of inducing regression of a tumor in a mammal in vivo comprising:
- contacting an undifferentiated cell ex vivo with an effective amount of an oligonucleotide substantially complementary to a region of human IGF-1 receptor RNA, wherein said IGF-1 receptor RNA expression is inhibited;
- and
- administering said undifferentiated cell to said mammal.
Dependencies are inferred from the claim text.