Method for treating cardiac malfunction
AssigneeThe General Hospital Corporation
InventorGarner Haupert, Jr.
Life of this patent
Estimating the term…
Abstract
A method of treating cardiac malfunction by administering a positive inotropic effect-producing amount of Hypothalamic Inhibitory factor (HIF).
Claims14
A composition which is purified to homogeneity and which is characterized by a mass spectrum depicted in FIG. 15B.
A composition which is purified to homogeneity and which, after naphthoylation, yields a CD spectrum of FIG. 10B, 13B or 14B.
A composition purified to homogeneity which, when applied to a CHP20P column, elutes from the column at about 84 minutes using a linear methanol gradient (0-100%) pumped at 2 ml/min. and which has a molecular weight of 584 g/mol.
A pharmaceutical composition which is purified to homogeneity, comprising a therapeutic amount of a Hypothalamic Inhibitory Factor wherein said Factor:
- (a) has a molecular weight of 584 g/mol;
- (b) is substituted by a rhamnoside;
- (c) elutes from a CHP20P column at about 84 minutes using a linear methanol gradient (0-100%) pumped at 2 m/min;
- (d) is a non-peptidic steroid;
- (e) specifically binds Na, K ATPase reversibly and with high affinity;
- and
- (f) wherein said Factor is not ouabain.
A composition which is purified to homogeneity and which is formed by a method comprising the steps of:
- (a) methanol/water extraction;
- (b) petroleum ether and chloroform extraction;
- (c) lipophilic gel chromatography in methanol;
- (d) ion-exchange chromatography;
- (e) chromatography using CHP20P resin;
- (f) affinity purification using SDS-extracted Na, K-ATPase;
- and
- (g) reverse-phase C18-HPLC using a linear gradient of acetonitrile/water.
A glycosidic, non-peptidic Hypothalamic Inhibitory Factor which is purified to homogeneity, having a molecular weight of 584 g/mol, and a pharmaceutically acceptable carrier, wherein said factor is not ouabain.
A composition consisting essentially of Hypothalamic Inhibitory Factor which is purified to homogeneity.
Dependencies are inferred from the claim text.