Process and device for the isolation of cell components, such as nucleic acids, from natural sources
AssigneeQiagen GmbH
InventorMetin Colpan
Life of this patent
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Abstract
A filtration process for the preparation of nucleic acids from natural sources is disclosed. The sources containing nucleic acids are lysed; the lysate is left to rest for some time; the resulting lysate passes a filter layer. The filter layer is selected from silica gel, aluminum oxide or packed diatomaceous earth, or interwoven or cemented non-wovens of glass fibers and silica gel. Other filter layers include cellulose, paper, compressed paper, and paper non-wovens. The fraction leaving the filter layer is then collected and the nucleic acid is subsequently isolated and purified from the collected fraction. Furthermore, the filter layer has not been modified with anion exchange groups.
Claims15
A filtration process for the preparation of plasmid-DNA from a microorganism, the plasmid-DNA being suitable for gene therapy, the process comprising the steps of:
- (a) lysing the microorganism, thereby effecting a lysate;
- (b) allowing the lysate to rest for at least one minute;
- (c) passing the resulting lysate through a filter layer of packed diatomaceous earth that has not been modified with anion exchange groups, having a particle size between 5 μm and 500 μm;
- and
- (d) collecting the fraction which elutes from the packed diatomaceous earth, optionally followed by isolating the plasmid-DNA from the collected fraction.
Dependencies are inferred from the claim text.