Method capable of increasing competency of bacterial cell transformation

A kit for transforming DNA into competent bacteria, said kit comprising one or more vials, each of said vials containing an aliquot of a preparation of competent bacteria, said preparation comprising isolated bacteria derived from Escherichia coli DH5α and additionally comprising F′ genetic material derived from Escherichia coli XL2,

• wherein said isolated bacteria exhibit a higher transformation efficiency than said Escherichia coli DH5α which does not contain said F′ genetic material derived from Escherichia coli XL2.

A process for producing an E. coli bacterium capable of an enhanced transformation efficiency, said process comprising

• (a) introducing F′ genetic material into an E. coli bacterium that does not contain F′ genetic material;
• and
• (b) recovering an E. Coli bacterium containing said F′ genetic material, wherein after said E. coli bacterium containing said F′ genetic material is rendered competent, said E. coli bacterium containing said F′ genetic material exhibits a higher transformation efficiency than said E. coli bacterium that does not contain F′ genetic material.

A process for preparing competent bacteria, said process comprising

• (a) introducing F′ genetic material into an E. coli bacterium that does not contain F′ genetic material;
• (b) recovering an E. coli bacterium containing said F′ genetic material, and
• (c) rendering said E. coli bacterium containing said F′ genetic material competent, wherein after said E. coli bacterium containing said F′ genetic material is rendered competent, said E. coli bacterium containing said F′ genetic material exhibits a higher transformation efficiency than said E. coli bacterium that does not contain F′ genetic material.