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AssigneeThe Secretary of State for Defence in Her Britannic Majesty's Government of the United Kingdom of Great Britain and Northern Ireland
InventorsStephen Nicklin, Christopher Edward French, Neil Charles Bruce, Emma Rachel Travis, Amrik Basran
Estimating the term…
An enzymically active cell free extract or membrane fraction capable of catalysing the removal of nitrite from hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) is provided. The cell free extract is produced by culturing a strain of Rhodococcus rhodochrous bacterium from nature. The strain designated 11Y has been deposited as NCIMB 40820. Methods of bioremediation using the enzymically active cell free extract so produced are also provided. Additionally there is provided a method for detecting the presence of RDX in a sample together with a biosensor for use in such a method. The nitrite produced in the enzyme catalysed reaction with RDX is detected, for example, colorimetrically, or alternatively a product of RDX degradation, such as formal behyde, is detected.
A biologically pure culture of Rhodoccous rhodochrous NCIMB 40820, and mutants thereof having all of the identifying charateristics of said strain, wherein said strain is capable of producing a protein having enzymic activity which degrades hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX).
Dependencies are inferred from the claim text.