DNA coding for mammalian L-asparaginase
AssigneeKabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
InventorsTakeshi Ario, Madoka Taniai, Kakuji Torigoe, Masashi Kurimoto
Life of this patent
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Abstract
Disclosed is a DNA coding for mammalian L-asparaginase. Transformants introduced with the DNA effectively produce desired amounts of mammalian L-asparaginase such as those from humans, guinea pigs, and mice. The DNA is also useful as a probe for screening a DNA coding for desired mammalian L-asparaginase.
Claims7
An isolated DNA encoding for mammalian L-asparaginase, which comprises nucleotide sequences encoding for either or both the amino acid sequences of SEQ ID NOs:1 and 2, and which is hybridizable to any of the nucleotide sequences of SEQ ID NOs:3 to 5 at 42° C. in a solution of 5 ×SSPE, 5×Denhardts solution, 0.5 w/v% SDS, and 100 μg/ml denatured salmon sperm DNA, wherein said mammalian L-asparaginase has an amino acid sequence having at least 70% of its residues identical to the corresponding residues of the amino acid sequence of SEQ ID NO:7.
Dependencies are inferred from the claim text.