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US 6,274,366 B1Granted
Enzymatically-active recombinant human β-tryptase and method of making same
AssigneePromega Corporation
InventorsMark A. Maffitt, Andrew L. Niles, Mary Haak-Frendscho
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Abstract
Disclosed is a method of expressing enzymatically-active, recombinant human β-tryptase in a yeast host cell, expression constructs which drive the production of enzymatically-active tryptase in transformed yeast, and genetically-engineered yeast containing the expression constructs and which express enzymatically-active human β-tryptase. Uses for the tryptase so produced are also disclosed.
Claims13
A DNA expression construct comprising, in 5′ to 3′ order:
- a promoter, the promoter operationally linked to a signal sequence, the signal sequence operationally-linked to a DNA sequence encoding human β-tryptase, and wherein the expression construct drives the expression of enzymatically-active human β-tryptase in yeast hosts transformed to contain the expression construct, wherein the DNA sequence encoding human β-tryptase is SEQ. ID. NO:
- 1.
A DNA expression construct comprising, in 5′ to 3′ order:
- a promoter selected from the group consisting of AOX1, GAP, MOX, FMD, ADH, LAC4, XPR2, LEU2, GAM1, PGK1, GAL7. GADPH, CYC1, and CUP1, the promoter operationally linked to a signal sequence, the signal sequence operationally-linked to a DNA sequence encoding human β-tryptase, the DNA sequence operationally linked to a terminator sequence, wherein the DNA sequence encoding human β-tryptase is SEQ. ID. NO:
- 1.
Dependencies are inferred from the claim text.