RGS-containing molecules and uses thereof
AssigneeMillennium Pharmaceuticals, Inc.
InventorsMartin R. Hodge, David Yowe
Life of this patent
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Abstract
Novel RGS polypeptides, proteins, and nucleic acid molecules are disclosed. In addition to isolated, full-length RGS proteins, the invention further provides isolated RGS fusion proteins, antigenic peptides, and anti-RGS antibodies. The invention also provides RGS nucleic acid molecules, recombinant expression vectors containing a nucleic acid molecule of the invention, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which an RGS gene has been introduced or disrupted. Diagnostic, screening, and therapeutic methods utilizing compositions of the invention are also provided.
Claims39
An isolated nucleic acid molecule selected from the group consisting of:
- a) a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:1, SEQ ID NO:3, nucleotides 160-864 of SEQ ID NO:1, nucleotides 134-838 of SEQ ID NO:3, the cDNA insert of the plasmid deposited with ATCC as Accession Number 207048, the DNA sequence obtained from the overlapping clones deposited with ATCC as Accession Numbers 207049 and 207050, or a complement thereof;
- and
- b) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:2, SEQ ID NO:4, an amino acid sequence encoded by the cDNA insert of the plasmid deposited with ATCC as Accession Number 207048, or an amino acid sequence encoded by the DNA sequence obtained from the overlapping clones deposited with ATCC as Accession Numbers 207049 and 207050.
A method for producing a polypeptide selected from the group consisting of:
- (a) a polypeptide comprising the amino acid sequence of SEQ ID NO:2, SEQ ID NO:4, an amino acid sequence encoded by the cDNA insert of the plasmid deposited with ATCC as Accession Number 207048, or an amino acid sequence encoded by the DNA sequence obtained from the overlapping clones deposited with ATCC as Accession Numbers 207049 and 207050;
- b) a polypeptide comprising a fragment of the amino acid sequence of SEQ ID NO:2, SEQ ID NO:4, an amino acid sequence encoded by the cDNA insert of the plasmid deposited with ATCC as Accession Number 207048, or an amino acid sequence encoded by the DNA sequence obtained from the overlapping clones deposited with ATCC as Accession Numbers 207049 and 207050, wherein the fragment comprises at least 30 contiguous amino acids of SEQ ID NO:2, SEQ ID NO:4, an amino acid sequence encoded by the cDNA insert of the plasmid deposited with ATCC as Accession Number 207048, or an amino acid sequence encoded by the DNA sequence obtained from the overlapping clones deposited with ATCC as Accession Numbers 207049 and 207050, wherein said figment has RGS activity;
- and
- c) a polypeptide having RGS activity, wherein the polypeptide is encoded by a nucleic acid molecule which hybridizes to a nucleic acid molecule comprising SEQ ID NO:1, SEQ ID NO:3, nucleotides 160-864 of SEQ ID NO:1, nucleotides 134-838 of SEQ ID NO:3, or a complement thereof under stringent conditions;
- by culturing a host cell which comprises a nucleic acid molecule encoding the polypeptides of a), b) or c) under conditions in which the polypeptide is produced.
An isolated nucleic acid molecule comprising a nucleic acid sequence which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:2.
An isolated nucleic acid molecule which encodes a fragment of a polypeptide comprising the amino acid sequence of SEQ ID NO:2, wherein the fragment has RGS activity and comprises at least 30 contiguous amino acids of SEQ ID NO:2.
An isolated nucleic acid molecule which encodes a polypeptide having RGS activity, wherein the nucleic acid molecule hybridizes to a nucleic acid molecule comprising SEQ ID NO:1, nucleotides 160-864 of SEQ ID NO:1, or a complement thereof, under stringent conditions, said stringent conditions comprising hybridization in 6×SSC at 42° C., followed by washing with 1×SSC at 55° C.
Dependencies are inferred from the claim text.