Method for providing green note compounds
OwnerRichard Barry Holtz, Michael Jay McCulloch +3inventors
InventorsRichard Barry Holtz, Michael Jay McCulloch, Stephen John Garger, Richard King Teague, Harriet Flannery Phillips
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Abstract
Green note compound, such as cis-3-hexen-1-ol, is provided by subjecting linolenic acid and a fresh watermelon foliage to shearing in the presence of an aqueous liquid and yeast. Enzymes within the plant material (i.e., lipoxygenase and hydroperoxide lyase) and the yeast act to cause the linolenic acid to be converted to green note alcohol at a relatively high yield. Green note compound can be provided naturally using a continuous or batch process.
Claims20
A method for providing cis-3-hexen-1-ol, the method comprising the steps of:
- a) providing unsaturated fatty acid;
- b) providing plant biomass having active levels of lipoxygenase and hydroperoxide lyase enzymes;
- c) providing active alcohol dehydrogenase;
- d) providing a mixture by simultaneously contacting the fatty acid, plant biomass and alcohol dehydrogenase in the presence of an aqueous liquid under conditions sufficient to:
- i) provide release of lipoxygenase and hydroperoxide lyase from the plant biomass, and
- ii) provide reaction of the fatty acid with the lipoxygenase, hydroperoxide lyase and alcohol dehydrogenase to provide cis-3-hexen-1-ol;
- e) collecting aqueous phase containing cis-3-hexen-1-ol, such aqueous phase containing greater than 400 ug cis-3-hexen-1-ol per gram of plant biomass provided in step b);
- and
- f) separating cis-3-hexen-1-ol from the aqueous phase.
Dependencies are inferred from the claim text.