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US 6,274,355 B1Granted
Immobilized maltogenic α-amylase and its use in the manufacture of a maltose-rich syrup
AssigneeRoquette Freres
InventorsPierrick Duflot, Catherine Fouache
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Abstract
The invention concerns an immobilized maltogenic α-amylase, which is adsorbed on particles of at least one porous substrate selected from the group consisting of phenolic resins, acrylic resins and polystyrene resins. The present invention also relates to the use of such an α-amylase for the preparation of a maltose-rich syrup.
Claims11
A method of manufacturing a maltose-rich syrup, comprising the successive steps of:
- (a) carrying out liquefaction of a starch milk;
- (b) carrying out saccharification of the liquefied starch milk in the presence of a β-amylase, and at least one debranching enzyme selected from the group consisting of pullulanases and isoamylases;
- (c) bringing the liquefied and saccharified starch milk into contact with an immobilized maltogenic α-amylase which is adsorbed on particles of at least one porous substrate having an average pore diameter of between 200 and 800 Å and a particle diameter of between 0.2 and 1.2 mm in order to obtain a syrup which is rich in maltose.
A method of manufacturing a maltose-rich syrup, comprising the steps of:
- (a) carrying out liquefaction of a starch milk;
- (b) carrying out saccharification of the liquefied starch milk in the presence of a β-amylase, and at least one debranching enzyme selected from the group consisting of pullulanases and isoamylases;
- (c) performing a molecular sieving of the liquefied and saccharified starch milk so as to obtain a maltose-enriched fraction and a maltose-depleted fraction;
- (d) bringing the said maltose-enriched fraction into contact with an immobilized maltogenic α-amylase which is adsorbed on particles of at least one porous substrate having an average pore diameter of between 200 and 800 Å and a particle diameter of between 0.2 and 1.2 mm in order to obtain a syrup which is rich in maltose.
1 more element
Dependencies are inferred from the claim text.