Biosynthetic genes for spinosyn insecticide production
AssigneeDow AgroSciences LLC
InventorsRichard H. Baltz, Kathryn P. Crawford, M. Christine Broughton, Krishnamurthy Madduri, Donald J. Merlo, Jan R. Turner, Patti J. Treadway, Clive Waldron
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Abstract
Spinosyn biosynthetic genes, spinosyn producing microorganisms transformed with the biosynthetic genes, methods using the biosynthetic genes to increase production of spinosyn insecticidal macrolides, and methods using the genes or fragments thereof to change the products produced by spinosyn-producing microorganisms.
Claims9
An isolated DNA molecule comprising a DNA sequence that encodes a spinosyn biosynthetic enzyme, wherein said enzyme is defined by an amino acid sequence selected from the group consisting of SEQ ID NOS 7-24, 26, 27, 29, and 33.
A transformed spinosyn-producing microorganism having spinosyn biosynthetic genes in its genome wherein at least one of the spinosyn biosynthetic genes, selected from spnN, spnO, spnP, spnQ, spnR, spnS, S. spinosa gdh , S. spinosa epi, and S. spinosa kre, is duplicated.
A transformed spinosyn-producing microorganism having spinosyn biosynthetic genes in its genome, wherein at least one of said genes has been disrupted by recombination with an internal fragment of that gene, the rest of said genes being operational to produce a spinosyn other than the one that would be produced if the disrupted gene were operational wherein the disrupted gene is selected from the group consisting of genes encoding spnN, spnO, spnP, spnQ, spnR, spnS.
Dependencies are inferred from the claim text.