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AssigneeKorea Advanced Institute of Science and Technology
InventorsSang Yup Lee, Zhaohui Xu, Jong Hyun Choi
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The present invention relates to expression vectors containing a gene encoding outer membrane protein C(OmpC) from Escherichia coli as a cell surface anchoring motif, more specifically, to expression vectors comprising a gene encoding OmpC which is designed to express a gene of foreign protein in fused form with OmpC on the cell surface of Escherichia coli, and a method for displaying the desired protein on the surface of the bacteria employing the OmpC as a cell surface anchoring motif. In accordance with the present invention, the desired protein can be expressed efficiently on the cell surface of bacteria so that the expressed protein can be applied to a variety of applications such as live vaccine development, peptide libraries screening, antibody production, environmental bioadsorbent, whole cell catalysis, and biosensor development.
An expression vector designed to express a foreign protein in fused form with an OmpC as a cell surface anchoring motif on the cell surface of a microorganism comprising:
A heavy metal remover which comprises a recombinant microorganism transformed with an expression vector comprising a gene encoding a poly-histidine linker peptide fused to the entire gene of E. coli outer membrane protein C(OmpC) as a cell surface anchoring motif.
Dependencies are inferred from the claim text.