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US 6,274,337 B1Granted
High throughput screening assay systems in microscale fluidic devices
AssigneeCaliper Technologies Corp.
InventorsJ. Wallace Parce, Anne R. Kopf-Sill, Luc J. Bousse
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Abstract
The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays. In particular, the devices and methods of the invention are useful in screening large numbers of different compounds for their effects on a variety of chemical, and preferably, biochemical systems.
Claims41
A method of screening a plurality of test compounds for an effect on a cell comprising:
- providing a body structure having at least two intersecting channels disposed therein, at least one of the two intersecting channels having at least one cross-sectional dimension in a range from 0.1 to 500 μm;
- flowing a first biological cell in a first channel;
- flowing at least a first test compound from a second channel into the first channel;
- contacting the first biological cell in the first channel with the at least first test compound and,
- detecting an effect of the at least first test compound on the first biological cell.
2 more elements
A method of determining whether a sample contains a compound capable of affecting a cell, comprising:
- providing a body structure having at least two intersecting channels disposed therein, at least one of tbe two intersecting channels having at least one cross-sectional dimension in a range from 0.1 to 500 μm;
- flowing a first cell in a first channel;
- flowing the sample from a second channel into the first channel;
- contacting the first cell in the first channel with the sample;
- and,
- detecting an effect of the sample on the cell.
3 more elements
Dependencies are inferred from the claim text.