L-AP4 sensitive glutamate receptors
AssigneesZymoGenetics, Inc., State of Oregon, Acting by and Through the Oregon State Board of Higher Education on Behalf of the Oregon Health Sciences University
InventorsThomas P. Segerson, J. Mark Kinzie, Eileen R. Mulvihill, Julie A. Saugstad, Gary L. Westbrook
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Abstract
Metabotropic receptor mGluR7 is identified and sequenced. The mGluR7 receptor subfamily mediates inhibition of transmitter release at selected glutamatergic synapses. The receptors, mGluR7-specific peptides and antibodies thereto are used to identify agonists and antagonists of G protein coupled glutamate receptor mediated neuronal transmitter release, as well as in methods of diagnosis and therapy.
Claims14
Isolated and purified metabotropic glutamate receptor protein which has the amino acid sequence shown in SEQ ID NO:2 or a polypeptide comprising at least 100 contiguous amino acids thereof.
An isolated polynucleotide molecule which codes for a metabotropic glutamate receptor protein which has the amino acid sequence shown in SEQ ID NO:2 or a polypeptide comprising at least 100 contiguous amino acids of said protein.
A DNA construct comprising the following operably linked elements:
- a transcriptional promoter;
- an isolated DNA molecule encoding a metabotropic glutamate receptor protein which has the amino acid sequence shown in SEQ ID NO:2 or a polypeptide comprising at least 100 contiguous amino acids of said protein;
- and
- a transcriptional terminator.
A cultured eukaryotic cell transformed or transfected with a DNA construct which comprises the following operably linked elements:
- a transcriptional promoter;
- an isolated DNA molecule encoding a metabotropic glutamate receptor protein which has the amino acid sequence shown in SEQ ID NO:2 or a polypeptide comprising at least 100 contiguous amino acids of said protein;
- and
- a transcriptional terminator.
A method for identifying a compound which alters metabotropic glutamate receptor mediated-metabolism, which comprises incubating the compound with eukaryotic cells which express recombinant metabotropic glutamate receptor protein which has the amino acid sequence shown in SEQ ID NO:2, wherein said metabotropic glutamate receptor protein is activated by L-2-amino-4-phosphonobutryic acid (L-AP4) to inhibit stimulation of intracellular cAMP production, and determining therefrom the effect of said compound on metabotropic glutamate receptor-mediated metabolism in the cells.
Dependencies are inferred from the claim text.