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US 6,274,313 B1Granted
Oligonucleotides with cationic phosphoramidate internucleoside linkages and methods of use
AssigneesPioneer-Hybrid International, Inc., University of Iowa Research Foundation
InventorsDaniel L. Weeks, John Dagle, John G. K. Williams
Life of this patent
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Abstract
This invention relates to the use of oligonucleotides with cationic phosphoramidate internucleoside linkages for the identification of nucleic acid under DNA—DNA duplex denaturing conditions.
Claims16
A method for hybridizing an oligonucleotide to denatured nucleic acid to form a duplex comprising the steps of:
- hybridizing a duplex-forming oligonucleotide comprising at least one cationic N,N,diethyl ethylenediamine-class phosphoramidate internucleoside linkage to single-stranded nucleic acid;
- and
- detecting hybridization of the oligonucleotide to the single-stranded nucleic acid.
A method for detecting a nucleic acid fragment comprising the steps of:
- (a) hybridizing, under DNA—DNA duplex denaturing conditions, an oligonucleotide comprising about 30% to 100% cationic N,N,diethyl ethylenediamine-class phosphoramidate internucleoside linkages to a sample of nucleic acid to form a hybridization complex;
- and
- (b) detecting said hybridization complex, wherein detecting the hybridization complex indicates the presence of the nucleic acid fragment in the sample of nucleic acid.
Dependencies are inferred from the claim text.