Method and nucleotide sequence for transforming microorganisms
AssigneesUniversity of Guelph, University of Stellenbosch
InventorsJandre Grobler, Aldis Krizus, Chauanpit Osothsilp-Deeknamakul, Isak S. Pretorius, Hendrick J. Jansen Van Vuuren, Ronald E. Subden
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Abstract
An isolated nucleic acid molecule is provided which contains a sequence which encodes a protein which mediates the uptake of L-malate, succinate, and malonate, and expression vectors and host cells containing the nucleic acid molecules. The nucleic acid molecules are used to transform cells for use in mediating malate, succinic acid or malonate uptake in particular malate uptake during the fermentation of wines.
Claims34
An isolated nucleic acid molecule comprising a sequence which encodes a eukaryotic malate permease from Schizosaccharomyces pombe which mediates the uptake of L-malate, succinate, and malonate.
An isolated nucleic acid molecule comprising
- (i) a nucleic acid sequence encoding a protein having the amino acid sequence shown in SEQ ID NO:
- 2;
- or
- (ii) nucleic acid sequences complementary to (i).
An isolated nucleic acid molecule comprising
- (i) a nucleic acid sequence as shown in SEQ ID NO:1, wherein T can also be U;
- (ii) nucleic acid sequences complementary to (i);
- or
- (iii) a nucleic acid encoding a malate permease and capable of hybridizing under stringent conditions to a nucleic acid of (i) wherein the stringent conditions comprise 6.0×sodium chloride/sodium citrate (SSC) at about 45° C., followed by a wash of 2.0×SSC at about 50° C.
An isolated nucleic acid molecule comprising a sequence encoding a truncation of a protein having the amino acid sequence shown in SEQ ID NO:
- 2 and having malate permease activity.
An isolated nucleic acid probe encoding at least 6 sequential amino acids from the sequence shown in SEQ.ID.NO.:2.
An isolated protein which is a truncation, of the protein which has the amino acid sequence as shown in SEQ.ID.NO.:2, which mediates the uptake into a cell of external L-malate, succinate and malonate and has malate permease activity.
Dependencies are inferred from the claim text.