US 6,274,307 B1 · Immunologically active peptides or polypeptides from the parvovirus B19

Abstract

Immunologically active peptides or polypeptides with a partial amino-acid sequence of the capsid proteins VP1 and VP2 of parvovirus B19 which permit tests to be carried out at low cost, sensitively and specifically for the determination of antibodies against human parvovirus B19 are made available. Short peptide sequences which, employed as antigen, serve to identify anti-B19 IgG-positive sera are identified. Furthermore, the production of these peptides using genetic engineering measures is disclosed. Other antigens which are produced by genetic engineering and which can be stably produced in a high yield in E.coli and subsequently purified therefrom are used as additional antigens for IgG detection. Finally, a set of antigens permits tests to be carried out to determine IgM antibodies against the virus. In addition, the components, produced by genetic engineering, of the surface proteins represent substances which can be used for prophylactic immunisation.

Claims20

Dependent claims

A test kit for the detection of antibodies against human parvovirus B 19, said test kit comprising:

A) a peptide or polypeptide consisting of an amino acid sequence selected from the group consisting of:
i) a partial amino acid sequence consisting of 8 to 50 consecutive amino acid residues of SEQ ID NO.:
15, said partial amino acid sequence being capable of reacting with an antibody specific for the capsid protein VP1 of parvovirus B19;
and
ii) an amino acid sequence selected from the group consisting of SEQ ID NO.:
1, SEQ ID NO.:
2, SEQ ID NO.:
3, SEQ ID NO.:
4, SEQ ID NO.:
5, SEQ ID NO.:
6, SEQ ID NO.:
7, SEQ ID NO.:
8, SEQ ID NO.:
9, SEQ ID NO.:
10, SEQ ID NO.:
11, SEQ ID NO.:
12, SEQ ID NO.:
13, SEQ ID NO.:
14, SEQ ID NO.:
16, SEQ ID NO.:
17, SEQ ID NO.:
18, SEQ ID NO.:
19, SEQ ID NO.:
20, SEQ ID NO.:
21;
and SEQ ID NO.:
22;
and
B) at least one indicator which makes it possible to detect a complex of said peptide or polypeptide and an antibody.

26 more elements

Dependencies are inferred from the claim text.

Immunologically active peptides or polypeptides from the parvovirus B19

Life of this patent

Immunologically active peptides or polypeptides from the parvovirus B19

Life of this patent

Abstract

Claims20