Carrier for extracting bacteria, method of extracting microorganism and method of assaying microorganisms
AssigneesSanyo Electric Co., Ltd., Society for Techno-Innovation of Agriculture, Forestry and Fisheries
InventorTakakazu Inoue
Life of this patent
Estimating the term…
Abstract
A carrier for extracting bacteria is formed by a material body having a plurality of pores with no DNA. The pores are 5 to 100 μm in mean pore diameter, and at least 5 μm in depth. Bacteria enter the pores of the carrier for extracting bacteria buried in a sampling site and inhabit therein. After a lapse of a prescribed time, the carrier for extracting bacteria is taken out and washed for removing large-sized foreign matter. Further, the carrier for extracting bacteria is crushed to liberate the bacteria.
Claims17
A method of extracting a microorganism comprising steps of:
- introducing a carrier for extracting a microorganism at a site for sampling microorganisms, said carrier being formed by a material body which has a plurality of pores and which does not comprise DNA;
- recovering said carrier for extracting a microorganism after a lapse of a prescribed time;
- and
- liberating said microorganisms from said recovered carrier for extracting a microorganism.
A method of assaying microorganisms comprising steps of:
- introducing a carrier at a site for sampling a group of microorganisms, the carrier being formed by a material body, wherein the body has a plurality of pores and does not comprise DNA;
- extracting the group of microorganisms by crushing said carrier, whereby said group of microorganisms is liberated from said carrier;
- amplifying DNA obtained from said group of microorganisms by polymerase chain reaction using a plurality of primers having different amplification probabilities, whereby amplified DNA fragments are obtained;
- classifying said amplified DNA fragments by a discrimination method;
- and
- discriminating said plurality of different microorganisms included in said group of microorganisms.
Dependencies are inferred from the claim text.