Method of preparing an extract potent in anti-inflammation and anti-platelet aggregation from Zingiber officinale and pharmaceutical compositions containing said extract

A method of preparing a product potent in anti-inflammation or in anti-platelet aggregation from rhizomes of Zingiber officinale comprising the following steps:

• a) preparing a crude liquid from rhizomes of Zingiber officinale;
• b) introducing the crude liquid to a reverse phase chromatography column, and eluting the column with water, a first eluent and a second eluent in sequence, said second eluent having a polarity weaker than that of the first eluent but stronger than that of chloroform, so that a first eluate resulting from elution of the first eluent and a second eluate resulting from elution of the second eluent are obtained;
• c) removing the first eluent from the first eluate by evaporation, so that a first concentrated eluate is obtained and is able to be used as the potent product;
• and
• d) removing the second eluent from the second eluate by evaporation, so that a second concentrated eluate is obtained and is able to used as the potent product;
• wherein step a) comprises steps i) to iv), or comprises step I), step I′), or step I″), wherein said steps i) to iv) are:
• i) shedding fresh rhizomes of Zingiber officinale and filleting the resulting mixture to obtain a filtrate and a residue;
• ii) extracting the filtrate with a first organic solvent, recovering the resulting extraction solution of the first organic solvent, and evaporating the first organic solvent from the extraction solution to obtain a first concentrated extraction solution;
• iii) extracting the residue with a second organic solvent, recovering the resulting extraction solution of the second organic solvent, and evaporating the second organic solvent from the extraction solution to obtain a second concentrated extraction solution;
• and
• iv) combining the first concentrated extraction solution and the second concentrated extraction solution to obtain the crude liquid;
• said step I) is:
• I) extracting powder of dried rhizomes of Zingiber officinale with the second organic solvent, recovering the resulting extraction solution of the second organic solvent, and evaporating the second organic solvent from the extraction solution to obtain the crude liquid;
• said step I′) is:
• I′) steam distilling powder of dried rhizomes of Zingiber officinale, and concentrating the resulting distillate by evaporation to obtain the crude liquid;
• and
• said step I″) is:
• I″) extracting powder of dried rhizomes of Zingiber officinale with supercritical CO2, recovering the resulting extraction solution of the supercritical CO2, and evaporating CO2 from the extraction solution to obtain the crude liquid.