Biologically active phytogenous proteoglycan and a method of making thereof
OwnerLudmila Chekanovskayainventor
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OwnerLudmila Chekanovskayainventor
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A biologically active phytogenous proteoglycan not having hemagglutinating activity via water solution obtained by disintegration of divisible plant cells from young potato plants, Solanum tuberosum of the Family Solanaceae, fractionated and concentrated to obtain a dry substance with a molecular mass of 8.0×105-2.5×106D and the mass percentages of the following elements: nitrogen 1.12-2.48%, carbon 39.93-44.42%, hydrogen 5.15-7.21%; the remaining is an ash component, which includes a polysaccharide chain consisting of residues of neutral sugars in the amount of 34.0-85.3% and in the following mass percentages: glucose 26.4-33.1%, galacturonic acid 19.0-25.1%, arabinose 1.7-4.4%, uronic acids 12.0-18.0%, rhamnose 1.2-10.0%, xylose 0.1-3.0%, mannose 0.1-5.0%, galactose 2.5-27.0%; and up to 15 ng of pro that consists of amino acids residues in the following quantities based on ng per 0.1 mg of proteoglycan: asparagine 126.0-146.0 ng, serine 139.0-159.0 ng, glutamine 263.0-283.0 ng, glycine 117.0-131.0 ng, alanine 80.0-100.0 ng, valine 76.0-96.0 ng, leucine 85.0-105.0 ng, lysine 62.0-85.0 ng, arginine 42.0-62.0 ng; and trace amounts of cysteine, isoleucine, histidine, phenylalanine, tyrosine, and threonine.
A biologically active phytogenous proteoglycan not having hemagglutinating activity via water solution obtained by disintegration of divisible plant cells from young potato plants, Solanum tuberosum of the Family Solanaceae, fractionated and concentrated to obtain a dry substance with a molecular mass of 8.0×105-2.5×106D and the mass percentages of the following elements:
A method of producing biologically active a phytogenous proteoglycan comprising the steps of shredding into mush-like condition a raw material from germinated potato bulbs of Solanum tuberosum of the Family Solanaceae, extracting with boiling water, whereby the ratio of the water to the raw material is 1.1:1.2, separating by filtration an extract into a solid and a liquid phase, fractionating the latter by either gel-chromatography or filtration to remove fractions with a molecular weight not exceeding 8.0×105D, concentrating the extract to obtain a dry powder.
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