Calcium independent cytosolic phospholipase A2/B enzymes
AssigneeGenetics Institute, Inc.
InventorsSimon Jones, Jin Tang
Life of this patent
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Abstract
The invention provides a novel calcium-independent cytosolic phospholipase A2/B enzyme, polynucleotides encoding the enzyme and methods for screening unknown compounds for anti-inflammatory activity mediated by the arachidonic acid cascade.
Claims13
A composition comprising a peptide comprising an amino acid sequence selected from the group consisting of:
- (a) the amino acid sequence of SEQ ID NO:17;
- (b) a fragment of the amino acid sequence of SEQ ID NO:17 having activity in a mixed micelle assay with 1-palmitoyl-2-[14C]-arachidonyl-phosphatidylcholine;
- (c) the amino acid sequence of SEQ ID NO:19;
- (d) a fragment of the amino acid sequence of SEQ ID NO:19 having activity in a mixed micelle assay with 1-palmitoyl-2-[14C]-arachidonyl-phosphatidylcholine;
- (e) the amino acid sequence of SEQ ID NO:21;
- (f) a fragment of the amino acid sequence of SEQ ID NO:21 having activity in a mixed micelle assay with 1-palmitoyl-2-[14C]-arachidonyl-phosphatidylcholine;
- (g) the amino acid sequence of SEQ ID NO:23;
- and
- (h) a fragment of the amino acid sequence of SEQ ID NO:23 having activity in a mixed micelle assay with 1-palmitoyl-2-[14C]-arachidonyl-phosphatidylcholine.
An isolated polypeptide comprising an amino acid sequence selected from the group consisting of:
- a) the amino acid sequence of SEQ ID NO:17;
- b) a fragment of the amino acid sequence of SEQ ID NO:17 having activity in a mixed micelle assay with 1-palmitoyl-2-[14C]-arachidonyl-phosphatidylcholine;
- c) the amino acid sequence of SEQ ID NO:19;
- d) a fragment of the amino acid sequence of SEQ ID NO:19 having activity in a mixed micelle assay with 1-palmitoyl-2-[14C]-arachidonyl-phosphatidylcholine;
- e) the amino acid sequence of SEQ ID NO:21;
- f) a fragment of the amino acid sequence of SEQ ID NO:21 having activity in a mixed micelle assay with 1-palmitoyl-2-[14C]-arachidonyl-phosphatidylcholine;
- g) the amino acid sequence of SEQ ID NO:23;
- and
- h) a fragment of the amino acid sequence of SEQ ID NO:23 having activity in a mixed micelle assay with 1-palmitoyl-2-[14C]-arachidonyl-phosphatidylcholine.
An isolated polypeptide encoded by a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:16, SEQ ID NO:18, SEQ ID NO:20, SEQ ID NO:22, the DNA insert of the plasmid deposited with ATCC as Accession Number 69948 or 69949, or a complement thereof.
An isolated polypeptide comprising the amino acid sequence of SEQ ID NO:17, SEQ ID NO:19, SEQ ID NO:21, SEQ ID NO:23, or an amino acid sequence encoded by the DNA insert of the plasmid deposited with ATCC as Accession Number 69948 or 69949.
An isolated naturally-occurring allelic variant of a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 17, SEQ ID NO:
- 19, SEQ ID NO:
- 21, SEQ ID NO:
- 23, or an amino acid sequence encoded by the DNA insert of the plasmid deposited with ATCC as Accession Number 69948 or 69949, wherein the allelic variant has activity in a mixed micelle assay with 1-palmitoyl-2-[14C]-arachidonyl-phosphatidylcholine, and is encoded by a nucleic acid molecule which hybridizes to a compliment of a nucleic acid molecule comprising SEQ ID NO:
- 16, SEQ ID NO:
- 18, SEQ ID NO:
- 20, or SEQ ID NO:
- 22 under stringent conditions.
Dependencies are inferred from the claim text.