Enhancer elements for increased translation in plant plastids
AssigneeCalgene, Inc.
InventorsKevin E. McBride, Jeffrey M. Staub
Life of this patent
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Abstract
Provided are methods for increasing the production of protein in a plant cell by transforming plastids of plant cells with a construct comprising a promoter functional in a plant plastid, a ribosome binding site, DNA sequence of interest and a transcription termination region, and growing plant cells comprising the transformed plastids under conditions wherein the DNA encoding sequence is transcribed in the plastid. Also provided are methods for increasing protein production by fusing a coding sequence to a gene of interest to a secondary protein for cleavage or targetting of the protein of interest within the plastid, whereby high levels of expression of protein is achieved.
Claims12
A method for increasing the production of a protein in a plant cell containing plastids, wherein said method comprises:
- transforming plastids of said plant cell with a construct comprising the following as operably joined components in the 5′ to 3′ direction of transcription
- a) a promoter functional in a plant plastid;
- b) a DNA sequence encoding a peptide that confers tolerance to the herbicide glyphosate;
- c) a transcription termination region;
- and
- d) a ribosome binding site from the T7 bacteriophage gene 10 joined to said promoter component;
- and
- growing plant cells comprising said transformed plastids under conditions wherein said DNA sequence is transcribed in said plastid.
Dependencies are inferred from the claim text.