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US 6,271,440 B1Granted
Plant regulatory proteins III
AssigneeThe Australian National University
InventorsFranz Jacques Gubler, John Viggo Jacobsen
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AssigneeThe Australian National University
InventorsFranz Jacques Gubler, John Viggo Jacobsen
Estimating the term…
The invention relates generally to genetic constructs which are useful in regulating the synthesis of malting enzymes in plants. More particularly, the present invention relates to nucleic acid molecules encoding gibberellin-regulated MYB polypeptides, for example the barley and rice GAMyb genetic sequences and transcriptionally-modulating parts and/or immunologically interactive parts thereof. The genetic sequences of the invention are introduced into plant cells, in particular monocotyledonous plant cells such as those derived from barley, wheat, maize, rye, rice or sorghum, where their expression in either the sense or antisense orientation modulates the expression of hydrolytic malting enzymes which are normally regulated by GAMYB polypeptides. The genetic sequences of the invention are therefore useful in the production of plants with altered malting properties.
An isolated nucleic acid molecule comprising a coding sequence encoding a polypeptide that regulates the expression of one or more gibberellin-regulated genes or a fully complementary nucleotide sequence thereto wherein said polypeptide has 88% or greater amino acid sequence identity overall to SEQ ID NOs, 2 or 4, wherein each of said amino acid identities is calculated using an algorithm that maximizes the number of identical residues in an alignment between two or more proteins and minimizes the number of gaps in said alignment.
A plant cell transformed with the isolated nucleic acid molecule according to claim 11.
An isolated nucleic acid molecule that encodes a polypeptide and comprises the protein-encoding nucleotide sequence set forth in SEQ ID NO:1 or SEQ ID NO:3 or a fully complementary nucleotide sequence thereto.
An antisense genetic construct comprising in the antisense orientation the Myb genetic sequence set forth in SEQ ID NO:1 or SEQ ID NO:3 or a region thereof sufficient to reduce expression of an endogenous Myb genetic sequence in a plant cell, tissue or organ in operable connection with a promoter sequence.
A genetic construct comprising the protein-encoding region of a gibberellin-regulated Myb genetic sequence placed operably in connection with a promoter in the sense orientation such that a polypeptide is capable of being expressed under the control of said promoter wherein the Myb genetic sequence is the barley GAMyb sequence (HvGAMyb) set forth in SEQ ID NO:
An isolated nucleic acid molecule comprising a coding sequence encoding a polypeptide that regulates the expression of one or more gibberellin-regulated genes or a fully complementary nucleotide sequence thereto wherein said polypeptide has 75% or greater amino acid sequence identity overall to SEQ ID NOs:
Dependencies are inferred from the claim text.