Transgenic pathogen-resistant plant
OwnerJürgen Logemann, Guido Jach +5inventors
InventorsJürgen Logemann, Guido Jach, Birgit Görnhardt, John Mundy, Jeff Schell, Peter Eckes, Ilan Chet
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Abstract
Transgenic pathogen-resistant organism whose genome contains at least two different genes under the control of active promoters with pathogen-inhibiting action. This organism is distinguished by a synergistic pathogen-inhibiting action. This action is evident particularly when the genes code for the gene products chitinese (ChiS, ChiG), glucanase (GluG), protein synthesis inhibitor (PSI) and antifungal protein (AFR).
Claims13
A transgenic plant comprising:
- (i) at least one DNA sequence, operably linked to a plant-functional promoter, said DNA sequence encoding a protein selected from the group consisting of:
- a ChiG protein comprising the sequence as set forth in SEQUENCE ID No. 10, a GluG protein comprising the sequence as set forth in SEQUENCE ID NO;
- 12, a PSI protein comprising the sequence as set forth in SEQUENCE ID NO;
- 5 or 7, and an AFP protein comprising the sequence as set forth in SEQUENCE ID NO. 3, and
- (ii) a DNA sequence encoding a ChiS protein which is the gene product of the sequence as set forth in SEQUENCE ID NO. 8, operably linked to a plant-functional promoter,
- wherein the plant is resistant to fungal attack.
A transgenic plant comprising:
- (i) at least one DNA sequence, operably linked to a plant-functional promoter, said DNA sequence encoding a protein selected from the group consisting of:
- a ChiG protein comprising the sequence as set forth in SEQUENCE ID NO. 10, a GluG protein comprising the sequence as set forth in SEQUENCE ID NO. 12, a PSI protein comprising the sequence as set forth in SEQUENCE ID NO. 5 or 7, and a ChiS protein which is the gene product of the sequence as set forth in SEQUENCE ID NO. 8, and
- (ii) a DNA sequence encoding an AFP protein comprising the sequence as set forth in SEQUENCE ID NO. 3, operably linked to a plant-functional promoter,
- wherein the plant is resistant to fungal attack.
A purified and isolated DNA molecule comprising:
- (i) at least one DNA sequence, operably linked to a plant-functional promoter, said DNA sequence encoding a protein selected from the group consisting of:
- a ChiG protein comprising the sequence as set forth in SEQUENCE ID NO. 10, a GluG protein comprising the sequence as set forth in SEQUENCE ID NO. 12, a PSI protein comprising the sequence as set forth in SEQUENCE ID NO. 5 or 7, and an AFP protein comprising the sequence as set forth in SEQUENCE ID NO. 3, and
- (ii) a DNA sequence encoding a ChiS protein which is the gene product of the sequence as set forth in SEQUENCE ID NO. 8.
A purified and isolated DNA molecule comprising
- (i) at least one DNA sequence, operably linked to a plant-functional promoter, said DNA sequence encoding a protein selected from the group consisting of:
- a ChiG protein comprising the sequence as set forth in SEQUENCE ID NO. 10, a GluG protein comprising the sequence as set forth in SEQUENCE ID NO. 12, a PSI protein comprising the sequence as set forth in SEQUENCE ID NO. 5 or 7, and a ChiS protein which is the gene product of the sequence as set forth in SEQUENCE ID NO. 8, and
- (ii) a DNA sequence encoding an AFP protein comprising the sequence as set forth in SEQUENCE ID NO. 3.
A process for the generation of a transgenic fungus-resistant plant, comprising transforming a plant with:
- (i) at least one DNA sequence, operably linked to a plant-functional promoter, said DNA sequence encoding a protein selected from the group consisting of:
- a ChiG protein comprising the sequence as set forth in SEQUENCE ID NO. 10, a GluG protein comprising the sequence as set forth in SEQUENCE ID NO. 12, a PSI protein comprising the sequence as set forth in SEQUENCE ID NO. 5 or 7, and an AFP protein comprising the sequence as set forth in SEQUENCE ID NO. 3, and
- (ii) a DNA sequence encoding a ChiS protein which is the gene product of the sequence as set forth in SEQUENCE ID NO. 8, operably linked to a plant-functional promoter.
A process for the generation of a transgenic fungus-resistant plant, comprising transforming a plant with:
- (i) at least one DNA sequence, operably linked to a plant-functional promoter, said DNA sequence encoding a protein selected from the group consisting of:
- a ChiG protein comprising the sequence as set forth in SEQUENCE ID NO. 10, a GluG protein comprising the sequence as set forth in SEQUENCE ID NO. 12, a PSI protein comprising the sequence as set forth in SEQUENCE ID NO. 5 or 7, and a ChiS protein which is the gene product of the sequence as set forth in SEQUENCE ID NO. 8, and
- (ii) a DNA sequence encoding an AFP protein comprising the sequence as set forth in SEQUENCE ID NO. 3, operably linked to a plant-functional promoter.
A transgenic plant, comprising 2 transgenes, each operably linked to a plant-functional promoter, said transgenes encoding a protein selected from the group consisting of:
- (i) a PSI protein comprising the sequence of set forth in SEQUENCE ID NO. 5 to 7, and
- (ii) an AFP protein comprising the sequence as set forth in SEQUENCE ID NO. 3.
Dependencies are inferred from the claim text.