Enhanced expression of transgenes
AssigneeBoard of Regents, The University of Texas System
InventorsRichard J. Cristiano, Dao Nguyen
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Abstract
The present invention provides for improved methods of gene transfer, both in vitro and in vivo. By treating neoplastic cells with a DNA-damaging agent prior to transduction with a transgene, the expression of the transgene is improved up to about 3-fold over the expression seen in the absence of the DNA-damaging agent treatment. This effect is not dependent on the tumor cell type, the method of DNA transduction or type of DNA-damaging agent. The effect is most dramatic when the transduction is performed about 1-3 days following treatment with the DNA-damaging agent.
Claims10
A method for enhancing the expression of a transgene in a target neoplastic cell in vivo comprising:
- (a) administering a DNA-damaging agent to a subject containing a target neoplastic cell;
- and
- (b) transferring said transgene into said target neoplastic cell between 2-4 days after said administering step;
- whereby expression of said transgene is enhanced as a result of the administering of said DNA-damaging agent to said target neoplastic cell.
Dependencies are inferred from the claim text.