Composition for use in fluorescence assay systems
AssigneeKalibrant Limited
InventorsDerek Adeyemi Palmer, Martin Thomas French
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Abstract
The present invention relates to a composition for use in fluorometry, a method of fluorometry and the use of a class of new reagents in fluorometry. The composition comprises a substrate which is a non-fluorescent derivative of a fluorophore, and a shifting reagent which shifts the absorbance wavelength maximum of the fluorophore which maximum is naturally above 450 nm. The shifting reagent is present in an amount predetermined to shift the maximum to a preset value.
Claims19
A composition comprising a substrate which is a non-fluorescent derivative of a fluorophore, and a shifting reagent which shifts the absorbance wavelength maximum of the fluorophore which maximum is naturally above 450 nm, the shifting reagent being present in an amount predetermined to shift the maximum to a preset value.
A method of assaying for a desired product in a sample comprising the steps of:
- a) combining the sample with a substrate and a shifting reagent, the substrate being a non-fluorescent derivative of a fluorophore and the shifting reagent is a reagent which shifts the absorbance wavelength maximum of the fluorophore;
- b) exposing the product of step a to a light source having a maximum intensity at a known excitation wavelength and the shifting agent is used in an amount predetermined to shift the absorbance wavelength maximum of the fluorophore to a value substantially the same as the known excitation wavelength.
A method of shifting the absorbance wavelength maximum of a fluorophore in a detection system, comprising exposing a non-fluorescent derivative of said fluorophore to a predetermined amount of a shifting reagent.
A kit for use in a fluorescence assay system, said kit comprising one or more substrates, each substrate being a non-fluorescent derivative of a fluorophore, and a shifting reagent in an amount predetermined to shift the absorbance wavelength maximum of at least one of the fluorophores to a preset value.
Dependencies are inferred from the claim text.