Regeneration of both plant tissues and transgenic plant tissues using a new plant hormone, 5-bromoindole-3-acetic acid
AssigneeInvitrogen Corporation
InventorsJhy-Jhu Lin, Jianqing Lan, Nacyra Assad-Garcia
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Abstract
The present invention describes the use of 5-bromoindole-3-acetic acid (5-B-IAA) as an auxin affecting plant cell growth. The invention relates to the use of 5-B-IAA compositions to affect growth in monocotyledonous as well as in dicotyledonous plants. The invention also describes the use of 5-B-IAA in plant growth affecting compositions for the regeneration of both plant tissues and transgenic plant tissues. Further, the invention provides plant growth affecting compositions comprising 5-B-IAA alone or in a mixture comprising one or more additional plant growth regulators, such as cytokinin, etc.
Claims22
A method for enhancing a plant growth response comprising the step of growing the plant in the presence of a growth-enhancing, effective amount of a composition comprising 5-bromoindole-3-acetic acid, or a salt, an ester or an amide derivative thereof, wherein said plant growth response is selected from the group consisting of:
- stimulating seed germination:
- breaking of dormancy:
- increasing wields;
- hastening ripening and color production in fruit;
- increasing flowering and fruiting;
- stimulating shoot formation:
- inducing callus development;
- inducing rooting;
- causing cell proliferation;
- increasing hardiness;
- increasing the dry weight content of plants or plant parts;
- and
- wherein said plant is selected from the group consisting of:
- a whole plant;
- a transformed plant;
- a part of a plant;
- a locus of a plant;
- a cell of a plant:
- a tissue of a plant;
- an explant;
- a seed or seeds of a plant;
- a suspension culture or a tissue culture of a plant;
- a culture of calli;
- a culture of protoplasts;
- a culture of embryos;
- a culture of organs;
- or a culture of organelles;
- such that an enhancement of said plant growth response occurs in said plant in the presence of said composition when compared to a corresponding plant in the absence of said composition.
Dependencies are inferred from the claim text.