Cell and tissue culture device with enhanced culture fluid flow

A cell and tissue culture device comprising at least a culture well (18-i) defining a chamber (22-i) able to receive cells or tissues to be grown, having first (2) and second (25) tanks, wherein at least one of these tanks is configured to receive a culture fluid, and connecting structure (20,23,24) coupled to said well and said first and second tanks so as to enable the culture fluid to flow from one tank to another via said well,

• wherein each tank (20,25) accommodates at least one flexible pocket (6,7;27,29), wherein at least one of the pockets of these tanks is able to receive the culture fluid and said device comprises at least a controller module (50,150) able to provide first or second sequences of external pressures to be applied on the pockets of first and second tanks, respectively, and pressurization equipment (38,40-49,50-56,61-63) configured for applying to said pockets the pressures defined by said first and second sequences provided by the controller module, so as to control culture fluid flow in said well.