Modified factor VIII cDNA
AssigneeAventis Behring GmbH
InventorsClaude Négrier, Jean Luc Plantier
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Abstract
A modified Factor VIII cDNA is described comprising deletion of the B-domain and insertion of at least one truncated Factor IX intron 1 in the Factor VIII cDNA. The modified Factor VIII cDNA may be used for the production of a high yield of Factor VIII in vitro. The modified Factor VIII cDNA may be incorporated into a vector for gene therapy.
Claims6
A modified Factor VIII cDNA comprising a deletion of the B-domain and insertion of the truncated Factor IX intron 1 (SEQ ID NO:
- 9) in one or more splice sites of the Factor VIII cDNA, wherein said cDNA encodes a polypeptide having Factor VIII activity.
A modified Factor VIII cDNA comprising a replacement of the B-domain with nucleotides encoding four arginines and insertion of the truncated Factor IX intron 1 (SEQ ID NO:
- 9) in one or more splice sites of the Factor VIII cDNA, wherein said cDNA encodes a polypeptide having Factor VIII activity.
Dependencies are inferred from the claim text.