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US 6,271,010 B1Granted
Cyclomaltodextrin glucanotransferase variants
AssigneeNovzymes A/S
InventorsCarsten Andersen, Bjarne Rønfeldt Nielsen, Lubbert Dijkhuizen, Bauke Dijkstra
Life of this patent
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Abstract
The present invention relates to variants of cyclomaltodextrin glucanotransferase of increased product specificity.
Claims23
A CGTase variant having an amino acid sequence which differs from the amino acid sequence of a parent CGTase, wherein the parent CGTase is a Thermoanaerobacter CGTase and the difference between the amino acid sequence of the CGTase variant and the amino acid sequence of the parent CGTase comprises one or more of the following:
- 47C;
- 47D;
- 47E;
- 47F;
- 47G;
- 47I;
- 47K;
- 47N;
- 47P;
- 47S;
- 47T;
- 47V;
- 47W;
- 47Y;
- 145D;
- 145H;
- 145I;
- 145N;
- 145Q;
- 145V;
- 146H, 146L;
- 146T;
- 146V;
- 146Y;
- 147C;
- 147E;
- 147N;
- 147Q;
- 196C;
- 196E;
- 196F;
- 196H;
- 196I;
- 196K;
- 196M;
- 196P;
- 196Q;
- 196R;
- 196T;
- 196V;
- 196W;
- 196Y;
- and
- 371C;
- 371F;
- 371H;
- 371K;
- 371M;
- 371R;
- 371T;
- 371W;
- wherein each position corresponds to the position of the amino acid sequence of the mature CGTase obtained from Bacillus circulans strain 251.
49 more elements
Dependencies are inferred from the claim text.