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US 6,271,003 B1Granted
Method for identifying cordyceps sinenis
AssigneesRuey-Shyang Hseu, Soon Lo, Shih-Jen Wang
InventorsRuey-Shyang Hseu, Chih-Shang Chen
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Abstract
The present invention is drawn to a method for identifying Cordyceps sinensis, by amplifying a specimen's 18S rRNA polymorphism by PCR using primer pair NS3 and NS6; digesting the PCR product with restriction enzyme Cfo I; and identifying a genuine Cordyceps sinensis specimen by determining the presence of a PCR product digestible with the restrictions enzyme Cfo I and a DNA fragment in the polymorphism of the specimen belonging to a specific DNA fragment in the polymorphism of Cordyceps sinensis.
Claims2
A method for identifying Cordyceps sinensis, which comprises
- extracting DNA from a Cordyceps specimen;
- amplifying the DNA encoding 18S rRNA by PCR wherein the DNA contains a restriction fragment length polymorphism and wherein the PCR is performed using primer pair NS3 and NS6;
- digesting the amplified DNA fragments with restriction enzyme Cfo I;
- determining the restriction fragment lengths of the digested DNA;
- and
- identifying the specimen as Cordyceps sinensis by the presence of polymorphic restriction fragments lengths of 570bp, 300bp and/or 50bp.
3 more elements
Dependencies are inferred from the claim text.