DNA coding for human cell surface antigen
AssigneeOsaka Bioscience Institute
InventorsShigekazu Nagata, Naoto Itoh, Shin Yonehara
Life of this patent
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Abstract
DNAs coding for human cell surface antigen (Fas or Fas antigen), vectors for expressing for said DNAs and transformants transfected with said vector are proveded. Fas is a polypeptide that exists in the surfaces of a variety of cells and is considered to be deeply concerned with the apoptosis of cells. The isolated Fas CDNA has an open reading frame that is capable of encoding a protein consisting of 335 amino acids. The mature Fas antigen is a protein consisting of 319 amino acids having a calculated molecular weight of about 36,000 and is constituted by an extracellular domain of 157 amino acids, a membrane-spanning domain of 17 amino acids, and a cytoplasmic domain of 145 amino acids.
Claims18
An isolated DNA which consists of a nucleotide sequence of bases 243 to 1199 of that shown in SEQ ID NO:
- 1.
An isolated DNA consisting of a nucleic acid sequence encoding for a Fas antigen which comprises an amino acid sequence from 1 to 157 of that shown in SEQ ID NO:
- 2 and is recognized by a mouse anti-Fas antibody having an activity to induce apoptosis in cells alone.
An isolated DNA coding for a Fas antigen which comprises an amino acid sequence selected from the group consisting of −16 to 319 and 1 to 319 of that shown in SEQ ID NO:
- 2.
An isolated DNA consisting of a nucleic acid sequence encoding for a polypeptide which comprises an amino acid sequence selected from the group consisting of −16 to 157, and 1 to 157, of that shown in SEQ ID NO:
- 2.
An isolated DNA which comprises a nucleotide sequence of bases 243 to 713, of that shown in SEQ ID NO:
- 1.
A method of producing a Fas9 antigen, which comprises culturing a transformant transformed by a vector which comprises a DNA coding for a Fas antigen which consists of the amino acid sequence selected from the group consisting of amino acids −16 to 319 and 1 to 319 of that shown in SEQ ID NO:
- 2.
A method for producing a polypeptide which is part of Fas antigen, which comprises culturing a transformant transformed by a vector which comprises a DNA consisting of a nucleic acid sequence encoding for the amino acid sequence selected from the group consisting of amino acid sequences of amino acids −16 to 157, and 1 to 157, of that shown in SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.