Histidine kinase of Staphylococcus aureus
AssigneeSmithKline Beecham Corporation
InventorsNicola Gail Wallis, Christopher Michael Traini, Anna Lisa Kosmatka, Lisa Kathleen Shilling, Richard Lloyd Warren
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Abstract
The invention provides Histidine kinase polypeptides and polynucleotides encoding Histidine kinase polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are methods for utilizing Histidine kinase polypeptides to screen for antibacterial compounds.
Claims17
An isolated polynucleotide segment comprising a first polynucleotide sequence or the full complement of the entire length of the first polynucleotide sequence, wherein the first polynucleotide sequence is selected from the group consisting of:
- (a) a polynucleotide consisting of nucleotides 893 to 2263 set forth in SEQ ID NO:1;
- and,
- (b) a nucleic acid sequence identical to the polynucleotide of (a) except that, over the entire length corresponding to the polynucleotide of (a), up to five nucleotides are substituted, deleted or inserted for every 100 nucleotides of the polynucleotide of (a);
- wherein the first polynucleotide sequence is not genomic DNA and wherein the first polynucleotide sequence detects Staphylococcus aureus by hybridization.
An isolated polynucleotide segment, comprising a first polynucleotide sequence or the fill complement of the entire length of the first polynucleotide sequence;
- wherein the first polynucleotide sequence hybridizes to the fill complement of a reference polynucleotide consisting of nucleotides 893 to 2263 set forth in SEQ ID NO:1;
- wherein the hybridization conditions include incubation at 42° C. in a solution comprising:
- 50% formamide, 5×SSC (150 mM NaCl, 15 mM trisodium citrate), 50 mM sodium phosphate (pH7.6), 5×Denhardt's solution, 10% dextran sulfate, and 20 micrograms/ml denatured, sheared salmon sperm DNA, followed by washing in 0.1×SSC at 65° C.;
- wherein the first polynucleotide sequence is identical to the reference polynucleotide except that, over the entire length corresponding to the reference polynucleotide, up to five nucleotides arc substituted, deleted or inserted for every 100 nucleotides of the reference polynucleotide;
- wherein the first polynucleotide sequence is not genomic DNA and wherein the first polynucleotide sequence detects Staphylococcus aureus by hybridization.
An isolated polynucleotide segment comprising a first polynucleotide sequence, wherein the first polynucleotide sequence encodes a polypeptide comprising SEQ ID NO:2;
- wherein the first polynucleotide sequence is not genomic DNA.
An isolated polynucleotide segment, comprising a first polynucleotide sequence, wherein the first polynucleotide sequence encodes a polypeptide consisting of SEQ ID NO:2.
Dependencies are inferred from the claim text.