Neuron-restrictive silencer factor proteins
AssigneeCalifornia Institute of Technology
InventorsDavid J. Anderson, Christopher J. Schoenherr
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Abstract
The present invention relates to neuron-restrictive silencer factor proteins, nucleic acids, and antibodies thereto.
Claims5
A recombinant isolated neuron-restrictive silencer factor (NRSF) protein, comprising a protein selected from the group consisting of proteins having the amino acid sequence shown in FIG. 6 (SEQ ID NO:40), having the amino acid sequence shown in FIG. 12 (SEQ ID NO:50), and functional fragments thereof, wherein a functional fragment is an NRSF protein which
- (a) is at least 250 amino acids in length;
- (b) comprises more than one zinc finger motif;
- (c) is capable of binding to a neuron restrictive silencer element (NRSE);
- and
- (d) is capable of suppressing or silencing the expression of a neuronal gene.
A recombinant isolated nucleic acid encoding a neuron-restrictive silencer factor (NRSF) protein, wherein said nucleic acid encodes a protein having the amino acid sequence shown in FIG. 6 (SEQ ID NO:40) or in FIG. 12 (SEQ ID NO:50).
An expression vector comprising transcriptional and translational regulatory nucleic acid operably linked to a nucleic acid having the sequence shown in FIG. 6 (SEQ ID NO:39) or in FIG. 12 (SEQ ID NO:49).
A host cell transformed with an expression vector wherein the expression vector comprises a nucleic acid with the sequence shown in FIG. 6 (SEQ ID NO:39) or in FIG. 12 (SEQ ID NO:49).
A method of producing an isolated neuron-restrictive silencer factor (NRSF) protein, comprising:
- (a) culturing a host cell transformed with an expressing vector comprising a nucleic acid encoding a neuron-restrictive silencer factor (NRSF) protein, wherein the protein is selected from the group consisting of proteins having the amino acid sequence shown in FIG. 6 (SEQ ID NO:40), having the amino acid sequence shown in FIG. 6 (SEQ ID NO:50), and functional fragments thereof, wherein a functional fragment encodes an NRSF protein which
- (i) is at least 250 amino acids in length;
- (ii) comprises more than one zinc finger motif;
- (iii) is capable of binding to a neuron restrictive silencer element (NRSE);
- and
- (iv) is capable of suppressing or silencing the expression of a neuronal gene;
- and
- (b) expressing said nucleic acid to produce a neuron-restrictive silencer factor (NRSF) protein, and
- (c) recovering said protein.
Dependencies are inferred from the claim text.