O-fucosyltransferase
AssigneeGenentech, Inc.
InventorsYang Wang, Michael W. Spellman
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Abstract
The present invention describes the identification, purification, recombinant production and characterization of novel O-fucosyltransferase enzymes.
Claims15
A method of glycosylating an epidermal growth factor domain of a polypeptide with an activated O-fucose moiety comprising contacting said polypeptide with an O-fucosyltransferase which comprises an amino acid sequence selected from the group consisting of SEQ ID NO:4, SEQ ID NO:5 and SEQ ID NO:3.
A method of glycosylating an epidermal growth factor domain of a polypeptide with an activated O-fucose moiety comprising contacting said polypeptide with a enzymatically active O-fucosyltransferase comprising an amino acid sequence selected from the group consisting of:
- 1. SEQ ID NO:4:
- 2. SEQ ID NO:5;
- 3. SEQ ID NO:3;
- 4. SEQ ID NO:4 with one conservative substitution;
- 5. SEQ ID NO:5 with one conservative substitution;
- and
- 6. SEQ ID NO:3 with one conservative substitution.
Dependencies are inferred from the claim text.