ELISA serodiagnosis of pig pleuropneumonia serotypes 5a and 5b

An enzyme-linked immunosorbent assay (ELISA) diagnostic kit for the assay of A. pleuropneumoniae serotype 5a and 5b antibodies in the serum of a pig comprising in separate packaging the following:

• a) a solid support having bound thereto a purified lipopolysaccharide A. pleuropneumaniae serotype 5 antigen for a specific binding to anti-A pleuropneumaniae serotypes 5a and 5b antibodies present in the serum of the pig, said bound antigen being stabilized for at least 25 weeks at 4° C. with phosphate buffered saline pH 7.2, containing BSA, 0.1% thimerosal and caprylic acid;
• b) a positive control vial of serum from a pig experimentally inoculated with a strain of A. pleuropneumoniae serotype 5;
• c) a negative control vial of pig serum from a A. pleuropneumoniae free herd;
• d) a weak positive control vial of serum from a pig experimentally inoculated with a strain of A. pleuropneumoniae serotype 5;
• e) a conjugate vial of pig anti-IgG immunoglobulins coupled to peroxidase;
• and
• f) a calorimetric composition consisting of 3,3′,5,5′-tetramethylbenzidine or 2,2′-azino-bis(3-ethylbenzthia-zoline-6-sulfonic acid).