Characterizing nucleic acid by mass spectrometry
AssigneeBrax Group Limited
InventorsGunter Schmidt, Andrew Hugin Thompson
Life of this patent
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Abstract
The present invention provides a method for analyzing a population of nucleic acid fragments each labeled with a mass label, which method comprises: i) ionizing the population; ii) sorting the ionized population in a mass spectrometer according to mass into sub-populations each containing at least one labeled fragment; iii) cleaving each sub-population to release the mass label associated with each labeled fragment; iv) determining the mass of each released mass label by mass spectroscopy; and v) assigning each mass label to its associated fragment.
Claims16
A method for analysing a population of nucleic acid fragments each labelled with a mass label, which method comprises:
- (i) Ionising the population;
- (ii) sorting the ionised population in a mass spectrometer according to mass into sub-populations each containing at least one labelled fragment;
- (iii) cleaving each sub-population in a mass spectrometer by collision induced dissociation, to release the mass label associated with each labelled fragment;
- (iv) determining the mass of each released mass label by mass spectroscopy;
- and
- (v) assigning each mass label to its associated fragment, thereby identifying the nucleic acid fragment.
Dependencies are inferred from the claim text.