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US 6,270,971 B1Granted
Methods for detecting chromosomal aberrations using chromosome-specific paint probes
AssigneeApplied Imaging Corporation
InventorsMalcolm A Ferguson-Smith, Johannes F Wienberg, Stefan Müller
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Abstract
The invention provides methods and reagents for detecting a chromosomal aberration in an animal chromosome or karyotype. One or more detectably-labeled chromosome-specific probes from a first animal species are hybridized to chromosomes of a second animal species. This results in a banding pattern that can be compared to the pattern found in a normal chromosome or karyotype.
Claims14
A method for detecting a chromosomal aberration in a human patient, comprising:
- (a) obtaining a composition comprising detectably-labeled chromosome-specific paints prepared from chromosomes or chromosome fragments of at least two different nonhuman animal species;
- wherein said detectably-labeled chromosome-specific paints are detectably labeled with at least two different labels;
- (b) hybridizing the paints of the composition to one or more chromosomes of the human patient, thereby producing a banding pattern;
- (c) detecting the banding pattern resulting from the hybridization;
- (d) comparing the banding pattern detected to a banding pattern characteristic of normal human chromosomes hybridized with said paints, and
- (e) detecting a difference between the banding pattern detected in (c) and the banding pattern characteristic of normal human chromosomes, wherein a difference is indicative of a chromosomal aberration in the human patient.
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