Recombinant antigen from the NS3 region of the hepatitis C virus
AssigneeRoche Diagnostics GmbH
InventorsChristoph Seidel, Ursula-Henrike Wienhues, Urban Schmitt, Manfred Motz, Michael Wiedmann, Barbara Upmeier, Erwin Soutschek
Life of this patent
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Abstract
The present invention concerns a polypeptide which is composed of the amino acids 1207±10 to 1488±10 of a hepatitis C virus and of less than 20 foreign amino acids and the use of this polypeptide as an antigen in an immunological test.
Claims15
A method for determining presence of hepatitis C virus specific antibodies in a sample, comprising contacting said sample with an isolated polypeptide, the amino acid sequence of which consists of (i) an amino acid sequence which is at least 90% identical to amino acids 14-295 of SEQ ID NO:
- 2 and (ii) an amino acid sequence of less than 20 amino acids which is not found in hepatitis C virus, wherein (ii) is concatenated to the N-terminus or to the C-terminus of the amino acid sequence of (i), and determining binding thereto as a determination of antibodies to hepatitis C virus in said sample.
A method for determining presence of hepatitis C virus specific antibodies in a sample, comprising contacting said sample with a polypeptide, the amino acid sequence of which consists of (i) an amino acid sequence which is at least 90% identical to a sequence of at least amino acids 1217-1478 and no more than amino acids 1197-1498 from an NS3 region of a hepatitis C virus and (ii) an amino acid sequence of less than 20 amino acids which is not found in hepatitis C virus and is concatenated to the N-terminus or the C-terminus of (i), and determining binding thereto as a determination of antibodies to hepatitis C virus in said sample.
A method for determining presence of hepatitis C virus specific antibodies in a sample, comprising contacting said sample with an isolated polypeptide, the amino acid sequence of which consists of (i) at least amino acids 21-282 of SEQ ID NO:
- 9, and no more than amino acids 1-302 of SEQ ID NO:
- 9, and (ii) an amino acid sequence of less than 20 amino acids which is not found in hepatitis C virus, wherein (ii) is concatenated to the N-terminus or to the C-terminus of the amino acid sequence of (i), and determining binding thereto as a determination of antibodies to hepatitis C virus in said sample.
Reagent kit useful in determining an antibody against hepatitis C virus, comprising at least two polypeptides, each of which has an amino acid sequence which consists of (i) at least amino acids 21-282 of SEQ ID NO:
- 9, and no more than amino acids 1-302 of SEQ ID NO:
- 9, and (ii) an amino acid sequence of less than 20 amino acids which is not found in hepatitis C virus, wherein (ii) is concatenated to the N-terminus or to the C-terminus of the amino acid sequence of (i), wherein at least one of said polypeptides is bound to or is bindable to a solid phase, and another of said polypeptides is labelled.
Reagent kit useful in determining antibodies against hepatitis C virus in a sample, comprising a polypeptide, the amino acid sequence of which consists of (i) at least amino acids 21-282 of SEQ ID NO:
- 9, and no more than amino acids 1-302 of SEQ ID NO:
- 9, and (ii) an amino acid sequence of less than 20 amino acids which is not found in hepatitis C virus, wherein (ii) is concatenated to the N-terminus or to the C-terminus of the amino acid sequence of (i), bound to orbindable to a solid phase, and a labelled antibody which specifically binds to said polypeptide.
Dependencies are inferred from the claim text.