Aqueous solvent based encapsulation of a bovine herpes virus type-1 subunit vaccine
AssigneePfizer Inc.
InventorsTully J. Speaker, H. Fred Clark, Charlotte A. Moser, Paul A. Offit, Manuel Campos, Patrick J. Frenchick
Life of this patent
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Abstract
A microencapsulated subunit component from bovine herpes virus-1 (BHV-1) is disclosed. Vaccines, kits, and methods for using the same to vaccinate a member of a bovine species are also disclosed.
Claims19
A microcapsule comprising an anisotropic Lewis salt membrane encapsulating an aqueous core and an immunogenic composition surrounded by the salt membrane, said salt membrane comprising a precipitate resulting from the interfacial reaction of spermine and alginic acid or the interfacial reaction of a water-soluble neutral salt of spermine and a water-soluble neutral salt of alginic acid, said immunogenic composition comprising a subunit component of bovine herpes virus-1 (3HV-1), which subunit component is SHV-1-gD consisting of the amino acid sequence of amino acid residues 19 to 355 of SEQ ID NO:
- 2.
A method of increasing the immunogenicity of BHV-1 -gD consisting of the amino acid sequence of amino acid residues 19 to 355 of SEQ ID NO:
- 2, comprising encapsulating said BHV-1-gD in a microcapsule comprising an anisotropic Lewis salt membrane encapsulating an aqueous core, said salt membrane comprising a precipitate resulting from the interfacial reaction of spermine and alginic acid or the interfacial reaction of a water-soluble neutral salt of spermine and a water-soluble neutral salt of alginic acid.
A vaccine for immunizing a member of a bovine species against BHV-1, comprising an immunogenic amount of microcapsules comprising an anisotropic Lewis salt membrane encapsulating an aqueous core and an immunogenic composition surrounded by the salt membrane, said salt membrane comprising a precipitate resulting from the interfacial reaction of spermine and alginic acid or the interfacial reaction of a water-soluble neutral salt of spermine and a water-soluble neutral salt of alginic acid, said immunogenic composition comprising BHV-1-gD consisting of the amino acid sequence of amino acid residues 19 to 355 of SEQ ID NO:
- 2;
- and a veterinarily acceptable carrier.
A method of vaccinating a member of a bovine species against BHV-1, comprising administering to the animal a vaccine comprising an immunogenic amount of microcapsules comprising an anisotropic Lewis salt membrane encapsulating an aqueous core and an immunogenic composition surrounded by the salt membrane, said salt membrane comprising a precipitate resulting from the interfacial reaction of spermine and alginic acid or the interfacial reaction of a water-soluble neutral salt of spermine and a water-soluble neutral salt of alginic acid, said immunogenic Composition comprising BHV-1-gD consisting of the amino acid sequence of amino acid residues 19 to 355 of SEQ ID NO:
- 2, and a veterinarily acceptable carrier.
A kit for vaccinating a member of a bovine species against BHV-1, comprising a first container comprising an immunogenic amount of microcapsules comprising an anisotropic Lewis salt membrane encapsulating an aqueous core and an immunogenic composition surrounded by the salt membrane, said salt membrane comprising a precipitate resulting from the interfacial reaction of spermine and alginic acid or the interfacial reaction of a water-soluble neutral salt of spermine and a water-soluble neutral salt of alginic acid, said immunogenic composition comprising BHV-1-gD consisting of the amino acid sequence of amino acid residues 19 to 355 of SEQ ID NO:
- 2;
- and a second container comprising a diluent.
Dependencies are inferred from the claim text.